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HANDS-ON COMPREHENSIVE CELL INJURY/CELL
DEATH IMAGING WORKSHOPS

Tbuilding2otal # of Participants per Workshop is Limited to 4 to Maximize Personalized Training

Location: Biomedical Diagnostics & Research, Inc., 625 S. Plumer Ave. (3 miles from the University of Arizona (U of A) next to the U of A Track & Field).

Biomedical Diagnostics & Research Inc., in conjunction with Dr. Claire M. Payne, Research Professor, University of Arizona, will be offering a 12-hour (all day Friday and Saturday morning) hands-on comprehensive workshop in the preparation of cultured cells for evaluation using immunofluorescence (new Quantum Dot technology) in conjunction with other fluorescent molecular probes, immunohistochemistry, Romanowsky-type dyes and transmission electron microscopy for evaluation of cell injury and cell death. This workshop is designed to prepare the participant with

  1. Easily implemented procedures that avoid common pitfalls
  2. workstation202How to get set-up in the laboratory using inexpensive equipment
  3. Evaluation of participant-prepared slides using fluorescence microscopy
  4. Evaluation of a participant-prepared negative contrast grid under the electron microscope (see viral structures)
  5. An understanding of the role of morphology in the evaluation of cell injury and cell death in conjunction with molecular techniques
  6. An understanding of the principles of confocal microscopy and the use of deconvolution/deblurring algorithms, and
  7. Pitfalls to avoid when ordering antibodies for different cell applications. To allow for maximum laboratory exposure to these techniques, there will be only 3 half-hour interactive lectures on
  • proper fixation of cultured cells for light, fluorescence and electron microscopy
  • principles of confocal microscopy, deconvolution/deblurring algorithms, electron microscopy, fluorescent photoconversion for electron microscopy, and
  • evaluation of methods for assessing apoptosis (see apoptotic cell) and other modes of cell death, including necrosis, mitotic arrest, mitotic catastrophe (see mitotic catastrophe) (e.g. aberrant mitoses, micronuclei formation), and “dark cell” death.

At the end of this workshop, the participant will have acquired the following skills:
  • Successfully immunostain cells which are prepared on coverslips
  • Understand the value of Alexafluor dyes and Quantum dots
  • Comprehend the values and drawbacks of FRET (Fluorescence Resonance Energy Transfer)
  • Acquire digital images of Romanowsky dye-stained cells for different modes of cell injury/cell death, including: apoptosis, necrosis, mitotic arrest and aberrant mitoses
  • Prepare a Giemsa-stained slide of apoptotic cells
  • Prepare cultured cells for transmission electron microscopy
  • Recognize different cellular stresses (e.g. endoplasmic reticulum stress, nucleolar segregation, mitochondrial damage) and the appearance of apoptotic cells using the transmission electron microscope.
  • Successfully interpret electron micrographs of various cell injuries and avoid the common pitfalls of interpretation.
  • Integrate the results from brightfield, electron and fluorescence microscopy with common molecular techniques used to study cell injury and cell death.
1a

Brightfield Microscope
2a

Fluorescent Microscope
3a

Transmission Electron Microscope

Core Facilities at the University of Arizona: These techniques will allow participants from the University of Arizona to successfully prepare cultured cells for the Imaging Facility, Arizona Research Laboratories (confocal microscopy and electron microscopy) and the Histology Core Facility (Cell Biology & Anatomy Department, College of Medicine).

Price per Participant: $765.00

(Price includes hand-out of detailed protocols, all laboratory supplies, hands-on use of brightfield, fluorescent and transmission electron miscroscopes, computerized image capture software), take home glass slides of participant-stained (immunofluoresence and Giemsa) apoptotic cells, poster demonstrating modes of cell death, and lunch).

*Dates of Imaging Workshops

Fall 2006:

9/08-9/09
9/22-9/23
10/06-10/07
10/20-10/21
11/03-11/04
11/17-11/18
12/01-12/02
12/15-12/16

*Registration is done on a first-come/first-serve basis

2007 Workshop Schedule: please inquire at bmdr@opus1.com

2006 Registration Form

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